ABSTRACT
Four viral intestinal pathogens such as bovine coronavirus (BCoV), bovine rotavirus (BRV), bovine viral diarrhea virus (BVDV) and bovine astrovirus (BoAstV) are the most common causes of adult cattle diarrhea. In this study, 797 samples were collected from January 2020 to February 2023. Among them, BCoV is major pathogen we detected and the positive rate was 48.18% (471/794). Farther more, among 307 diarrhea samples and 487 asymptomatic samples, 159 and 312 were BCoV positive, respectively. The results reveal that BCoV is associated with bovine diarrhea (p=0.001). In addition, we also amplified and analyzed Hemagglutinin-esterase (HE), Spike (S) and whole genome of partially positive sample. Getting 8 HE complete sequence, 8 S complete sequence and 5 whole genomes. The molecular characterization provides reveal that 5 strains were branched Chinese strains, Japan and part of American strains in GⅡb，and Korea strains branched in GⅡa. BCoV-Neimeng S in this study was mutated 143H/A to 143R, which is uncommon in S gene. As a residue on the side chain of the core binding site of S1-NTD, this mutation changed the tertiary structure and may be possible to affect the glycoside binding spectrum. The noteworthy that BCoV-SJZ2 and BCoV-YZ1 have four amino acid insertions on HE, which is the same as SWUN/A10/2018. We also analysis 4.8kDa and 4.9kDa non-structural protein large-scale truncation and membrane protein mutation. This article will discuss epidemiology and genome analysis.